Background: Staphylococcus aureus more than any other human pathogen is a better model for the study of the\nadaptive evolution of bacterial resistance to antibiotics, as it has demonstrated a remarkable ability in its response\nto new antibiotics. This study was designed to investigate the in vitro transfer of mecA gene from methicillin\nresistant S. aureus to methicillin susceptible S. aureus.\nResult: The recipient transconjugants were resistant to erythromycin, cefpodoxime and were mecA positive. PCR\namplification of mecA after mix culture plating on Luria Bertani agar containing 100 �¼g/mL showed that 75% of the\ndonor and 58.3% of the recipient transconjugants were mecA positive. Additionally, 61.5% of both the donor cells\nand recipient transconjugants were mecA positive, while 46.2% and 41.75% of both donor and recipient\ntransconjugants were mecA positive on LB agar containing 50 �¼g/mL and 30 �¼g/mL respectively.\nConclusion: In this study, the direction of transfer of phenotypic resistance as well as mecA was observed to have\noccurred from the donor to the recipient strains. This study affirmed the importance of horizontal transfer events in\nthe dissemination of antibiotics resistance among different strains of MRSA.
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